Kit for quickly detecting sinotype deletional thalassemia based on hydrolysis probe method

The invention discloses a kit for quickly detecting sinotype deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R a...

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Bibliographische Detailangaben
Hauptverfasser:	PANG WANRONG, SUN LEI, LONG JU
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a kit for quickly detecting sinotype deletional thalassemia based on a hydrolysis probe method. The kit comprises a pair of primers DBT-F and DBT-R amplifying DBT alleles in beta-globin gene clusters and a fluorescent probe DBT-Prob for specific detection of DBT-F and DBT-R amplification products, wherein the DBT-F is 5'-GGGTGAGGAACAATTGAA AC-3', the DBT-R is 5'-ACCACATCCCTAACACAAC-3', and the DBT-probe is 5'-ROX-ACTATCA CACCCTGCTTACCTCTGATGGA-BHQ-2-3'. The kit can be used for achieving detection of DBT deletional thalassemia alleles through single-tube one-time reaction and has high sensitivity, stability and accuracy and higher specificity.