Technology of producing monosodium glutamate from potato starch
The invention discloses a technology of producing monosodium glutamate from potato starch. The technology comprises the following steps: adding moderate-temperature alpha-diastase, xylanase, 0.2% beta-glucanase and saccharifying enzyme to obtain enzymatic hydrolysate; configuring fermentation medium...
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Zusammenfassung: | The invention discloses a technology of producing monosodium glutamate from potato starch. The technology comprises the following steps: adding moderate-temperature alpha-diastase, xylanase, 0.2% beta-glucanase and saccharifying enzyme to obtain enzymatic hydrolysate; configuring fermentation medium, sterilizing the fermentation medium and cooling the fermentation medium to 33 DEG C; selecting and inoculating corynebacterium crenatum B9 in the fermentation medium, wherein the inoculum size is 1%, then fermenting at 38 DEG C and cultivating for 29-31 hours; after fermentation, extracting and refining the extract to prepare the monosodium glutamate. The monosodium glutamate prepared according to the technology has a purity of higher than 99.2% and completely satisfies the national requirements of salt-free monosodium glutamate. According to the technology, the corynebacterium crenatum B9 strain can be directly inoculated to ferment; moreover, the technology is high in fermentation efficiency, short in fermentation time and economical in cost. The salt-free monosodium glutamate of the invention is prepared from potato starch and opens up a new direction in the monosodium glutamate production industry. |
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