Bearded iris rooting culturing medium and method
The invention discloses a bearded iris rooting culturing medium and method. The culturing medium is characterized in that MS serves as a minimal culturing medium, 30 g/L to 40 g/L of white granulated sugar, 6 g/L to 7 g/L of agar and 0.2 mg/L to 1.0 mg/L of paclobutrazol are added, and the pH ranges...
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Zusammenfassung: | The invention discloses a bearded iris rooting culturing medium and method. The culturing medium is characterized in that MS serves as a minimal culturing medium, 30 g/L to 40 g/L of white granulated sugar, 6 g/L to 7 g/L of agar and 0.2 mg/L to 1.0 mg/L of paclobutrazol are added, and the pH ranges from 5.6 to 5.8. After induction rooting is carried out on bearded iris adventitious buds through the culturing medium, rooting is started after inoculation is carried out for 5 d, the rooting time is 10 d to 13 d earlier than that obtained through a traditional rooting culturing medium, the rooting rate is increased by 24.26% to 43.33%, transplanting time is advanced by 22 d to 26 d, the transplanting survival rate is increased by 18.65% to 28.34%, the rotting rate is reduced by 20.62% to 32.36%, the rooting culturing time of bearded irises is remarkably shortened, in-vitro rapid propagation efficiency is greatly improved, the use ratio of culturing space is greatly increased, a plant cluster is robust, tall and straight, a root system is thick and solid, leaves are green, and overall resistance is improved. |
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