Method and kit for detecting high-risk HPV (human papillomavirus) E6/E7 mRNA (messenger ribonucleic acid) by ligase

The invention relates to a method and a kit for detecting high-risk HPV (human papillomavirus) E6/E7 mRNA (messenger ribonucleic acid) by ligase. The method is characterized in that specific connection primers are connected into a cDNA (complementary deoxyribonucleic acid) fragment complementary with a target sequence of the HPV E6/E7 mRNA under the action of the ligase, and the successfully connected cDNA fragment can be amplified by a pair of universal primers in a later fluorescent PCR (polymerase chain reaction) system, in other words, eighteen high-risk HPVs capable of causing cervical cancer can be detected in the same amplification system by the single fluorescent PCR technology, so that the problems, such as low amplification efficiency and sensitiveness, caused by excessive primers are solved effectively. The aim of HPV E6/E7 mRNA detection is to screen out high-risk groups in HPV-infected people groups, so that the method has more significance in clinical diagnosis as compared with existing HPV genome L1 distinguishing type detection methods.