Method for identifying varieties of rescue grass by utilizing SRAP molecular marking
The invention discloses a method for identifying varieties of rescue grass by utilizing SRAP molecular marking. According to the method, 14 pairs of SRAP primers (SEQ ID NO.1-NO.14) are utilized for PCR amplification of 10 common varieties of rescue grass, values of 1 and 0 are assigned to 99 genera...
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Sprache: | eng |
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Zusammenfassung: | The invention discloses a method for identifying varieties of rescue grass by utilizing SRAP molecular marking. According to the method, 14 pairs of SRAP primers (SEQ ID NO.1-NO.14) are utilized for PCR amplification of 10 common varieties of rescue grass, values of 1 and 0 are assigned to 99 generated difference sites to establish fingerprints of the varieties, and the fingerprints are utilized for variety authenticity identification. The method adopts the SRAP molecular marking technology, so that the varieties of rescue grass can be correctly identified by more sensitive and efficient means of identification; compared with routine morphology inspection, the method has the characteristics of being more stable and reliable in identification result, rapid, simple, intuitional and practical. |
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