CDNA of Freesia refracta Klatt flavanonol-4-reductase genes
The new cDNA of three dihydroflavonol-4-reductase (DFR) genes is obtained through cloning from red flower Freesia refracta Klatt petals for the first time in the invention, and the nucleotide sequence of the cDNA and an amino acid sequence obtained from the nucleotide sequence are determined. Gene e...
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creator | WANG LI GAO XIANG LIU XINGXUE CAI XINQUAN |
description | The new cDNA of three dihydroflavonol-4-reductase (DFR) genes is obtained through cloning from red flower Freesia refracta Klatt petals for the first time in the invention, and the nucleotide sequence of the cDNA and an amino acid sequence obtained from the nucleotide sequence are determined. Gene expression of the three genes has obvious correlation with anthocyanin synthesis in the coloring process of Freesia refracta Klatt flowers; and additionally, after eukaryotic expression vectors of the three FhDFRs are transferred to Arabidopis thaliana tt3 mutants, phenotypes of seedlings and seed coats are recovered. |
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Gene expression of the three genes has obvious correlation with anthocyanin synthesis in the coloring process of Freesia refracta Klatt flowers; and additionally, after eukaryotic expression vectors of the three FhDFRs are transferred to Arabidopis thaliana tt3 mutants, phenotypes of seedlings and seed coats are recovered.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2015</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151202&DB=EPODOC&CC=CN&NR=105112429A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20151202&DB=EPODOC&CC=CN&NR=105112429A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG LI</creatorcontrib><creatorcontrib>GAO XIANG</creatorcontrib><creatorcontrib>LIU XINGXUE</creatorcontrib><creatorcontrib>CAI XINQUAN</creatorcontrib><title>CDNA of Freesia refracta Klatt flavanonol-4-reductase genes</title><description>The new cDNA of three dihydroflavonol-4-reductase (DFR) genes is obtained through cloning from red flower Freesia refracta Klatt petals for the first time in the invention, and the nucleotide sequence of the cDNA and an amino acid sequence obtained from the nucleotide sequence are determined. Gene expression of the three genes has obvious correlation with anthocyanin synthesis in the coloring process of Freesia refracta Klatt flowers; and additionally, after eukaryotic expression vectors of the three FhDFRs are transferred to Arabidopis thaliana tt3 mutants, phenotypes of seedlings and seed coats are recovered.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2015</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLB2dvFzVMhPU3ArSk0tzkxUKEpNK0pMLklU8M5JLClRSMtJLEvMy8_Lz9E10S1KTSkFShWnKqSn5qUW8zCwpiXmFKfyQmluBkU31xBnD93Ugvz41OKCxGSgqpJ4Zz9DA1NDQyMTI0tHY2LUAAAfSi5W</recordid><startdate>20151202</startdate><enddate>20151202</enddate><creator>WANG LI</creator><creator>GAO XIANG</creator><creator>LIU XINGXUE</creator><creator>CAI XINQUAN</creator><scope>EVB</scope></search><sort><creationdate>20151202</creationdate><title>CDNA of Freesia refracta Klatt flavanonol-4-reductase genes</title><author>WANG LI ; GAO XIANG ; LIU XINGXUE ; CAI XINQUAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN105112429A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2015</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG LI</creatorcontrib><creatorcontrib>GAO XIANG</creatorcontrib><creatorcontrib>LIU XINGXUE</creatorcontrib><creatorcontrib>CAI XINQUAN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG LI</au><au>GAO XIANG</au><au>LIU XINGXUE</au><au>CAI XINQUAN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>CDNA of Freesia refracta Klatt flavanonol-4-reductase genes</title><date>2015-12-02</date><risdate>2015</risdate><abstract>The new cDNA of three dihydroflavonol-4-reductase (DFR) genes is obtained through cloning from red flower Freesia refracta Klatt petals for the first time in the invention, and the nucleotide sequence of the cDNA and an amino acid sequence obtained from the nucleotide sequence are determined. Gene expression of the three genes has obvious correlation with anthocyanin synthesis in the coloring process of Freesia refracta Klatt flowers; and additionally, after eukaryotic expression vectors of the three FhDFRs are transferred to Arabidopis thaliana tt3 mutants, phenotypes of seedlings and seed coats are recovered.</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
title | CDNA of Freesia refracta Klatt flavanonol-4-reductase genes |
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