$CDNA of Freesia refracta Klatt flavanonol-4-reductase genes$

CDNA of Freesia refracta Klatt flavanonol-4-reductase genes

The new cDNA of three dihydroflavonol-4-reductase (DFR) genes is obtained through cloning from red flower Freesia refracta Klatt petals for the first time in the invention, and the nucleotide sequence of the cDNA and an amino acid sequence obtained from the nucleotide sequence are determined. Gene e...

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Bibliographische Detailangaben
Hauptverfasser:	WANG LI, GAO XIANG, LIU XINGXUE, CAI XINQUAN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The new cDNA of three dihydroflavonol-4-reductase (DFR) genes is obtained through cloning from red flower Freesia refracta Klatt petals for the first time in the invention, and the nucleotide sequence of the cDNA and an amino acid sequence obtained from the nucleotide sequence are determined. Gene expression of the three genes has obvious correlation with anthocyanin synthesis in the coloring process of Freesia refracta Klatt flowers; and additionally, after eukaryotic expression vectors of the three FhDFRs are transferred to Arabidopis thaliana tt3 mutants, phenotypes of seedlings and seed coats are recovered.