Recombinant river crab Esflol protein with binding activity on CHH and application

The invention discloses a recombinant river crab Esflol protein with binding activity on CHH and application. The protein has 426 amino acids which are as shown in SEQ ID NO. 1. A theoretical isoelectric point of the coded protein is 9.150, and the molecular weight is 47.18kD; and the protein has a nucleotide sequence which is shown in the SEQ ID NO. 1, and overall length of an open reading frame (ORF) in a corresponding gene sequence is 1281bp. An experiment result shows that a deduction is further verified by an immunohistochemical result of a river crab hepatopancreas tissue slice. Moreover, gene sequence analysis shows that the Esflol protein contains two possible cAMP or cGMP cyclin-dependent kinases phosphorylation sites, and the result prompts that the Esflol possibly participates in a signal channel mediated by cAMP/cGMP. The result proves that the Esflol is a link in the signal channel which is regulated and controlled by the CHH. The recombinant river crab Esflol protein with the binding activity on the CHH is peculiarly combined to the CHH, and nutritive value and flavor of hepatopancreas of cooked crabs are improved.