Method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis

The invention provides a method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis. The novel method for preparing crude phycobiliprotein and crude polysaccharide simultaneously comprises steps as follows: fresh and wet porphyra is taken as a raw material, harveste...

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Hauptverfasser: ZHANG JUNJIE, WANG LINGZHAO, PAN SAIKUN, LYU LINGLING, LIANG JUNJIE, DU YUNJIAN, SHU LIUQUAN, WANG SHUJUN, YAO XINGCUN, SU LIN
Format: Patent
Sprache:eng
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Zusammenfassung:The invention provides a method for preparing crude phycobiliprotein and crude polysaccharide from porphyra yezoensis. The novel method for preparing crude phycobiliprotein and crude polysaccharide simultaneously comprises steps as follows: fresh and wet porphyra is taken as a raw material, harvested porphyra yezoensis is washed, drained and subjected to centrifugal dewatering processing, and mixing swelling, wet-method ultrafine grinding performed by a colloid mill combined homogenizer, salting-out and other procedures are performed. According to the method, drying processing of the fresh and wet porphyra in the preparation process of phycobiliprotein is eliminated, a prepared crude phycobiliprotein product contains 34.3%-38.9% of phycobiliprotein on the dry basis, and the yield of phycobiliprotein ranges from 4.59% to 4.71%. The method is suitable for industrial application, the crude phycobiliprotein product prepared from porphyra can be taken as a preparation raw material of high-purity porphyra phycobiliprotein, and the prepared crude polysaccharide can be taken as a preparation raw material of polysaccharide.