Camellia oleifera pollen ultra low temperature storage method

The present invention relates to a camellia oleifera pollen ultra low temperature storage method, which comprises: 1, dewatering: placing collected camellia oleifera pollen into a constant temperature box to dry; 2, pre-freezing: pre-freezing the obtained camellia oleifera pollen; 3, freezing: pouri...

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Bibliographische Detailangaben
Hauptverfasser: CHANG WEIXIA, YAO XIAOHUA, LONG WEI
Format: Patent
Sprache:eng
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Zusammenfassung:The present invention relates to a camellia oleifera pollen ultra low temperature storage method, which comprises: 1, dewatering: placing collected camellia oleifera pollen into a constant temperature box to dry; 2, pre-freezing: pre-freezing the obtained camellia oleifera pollen; 3, freezing: pouring the pre-frozen camellia oleifera pollen into liquid nitrogen so as to be stored; and 4, thawing: thawing the stored camellia oleifera pollen. According to the present invention, with the camellia oleifera pollen ultra low temperature storage method, the pollen aging can be effectively inhibited, the respiration rate and the enzyme activity can be reduced, the camellia oleifera pollen life can be prolonged, the cross breeding difficulty caused by the non-meeting flowering seasons among the camellia oleifera species can be overcome, the vitality is up to 47.05% after the camellia oleifera pollen is stored in liquid nitrogen for six months, the drying time is short, the pollen viability is ensured, the storage is easily achieved, the drying method input cost, the pre-freezing cost and the thawing cost are low, the operation is simple, the hybridization viability of the camellia oleifera pollen can be effectively ensured, and the important promotion value is provided for the camellia oleifera cross breeding and production practice.