Primer group, kit and method for identifying salmonella pullorum and salmonella gallinarum

The invention discloses a primer group, a kit and a method for identifying salmonella pullorum and salmonella gallinarum. The primer group comprises two pairs of specific primers, wherein the two pairs of specific primers are respectively primer 1 and primer 2 as well as primer 3 and primer 4, where...

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Bibliographische Detailangaben
Hauptverfasser:	TAO ZHIYUN, ZHU CHUNHONG, SHU JINGTING, GONG JIANSEN, SHAN YANJU, SONG WEITAO, XU WENJUAN, LIU NIANHUA, JI GAIGE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a primer group, a kit and a method for identifying salmonella pullorum and salmonella gallinarum. The primer group comprises two pairs of specific primers, wherein the two pairs of specific primers are respectively primer 1 and primer 2 as well as primer 3 and primer 4, wherein the primer 1 is as shown in SEQ ID NO: 1, the primer 2 is as shown in SEQ ID NO: 2, the primer 3 is as shown in SEQ ID NO: 3 and the primer 4 is as shown in SEQ ID NO: 4; the kit contains the primer group; and the primer group is used for detecting to-be-detected bacteria by virtue of double PCR amplification. The primer group for identifying salmonella pullorum and salmonella gallinarum disclosed by the invention is strong in detection reaction specificity; through a PCR reaction, the salmonella pullorum and the salmonella gallinarum can be simultaneously detected from a sample, with high detection reaction sensitivity; and a detection process is rapid and efficient, and suitable for mass detection.