Species specificity primer pair, kit and method for fast identifying Bactrocera cucurbitae

The invention provides a species specificity primer pair, kit and method for fast identifying Bactrocera cucurbitae. The sequence of the specific primer GF85 of the species specificity primer pair is 5'-TCATCGTTACGGCTCAAGCAT-3'. The sequence of the specific primer GR531 of the species spec...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	YU WENTAO, WU ZHIYI, LIN YANGWU, YU YUN, CHEN ZHIMING, CHEN SHAOPING, HUANG ZHEN, LOU DINGFENG, GUO QIONGXIA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a species specificity primer pair, kit and method for fast identifying Bactrocera cucurbitae. The sequence of the specific primer GF85 of the species specificity primer pair is 5'-TCATCGTTACGGCTCAAGCAT-3'. The sequence of the specific primer GR531 of the species specificity primer pair is 5'-CCTTGGCTAATACAGGTAGAGA-3'. The species specificity primer pair has the advantages that the species specificity primer pair is more specific, is only capable of amplifying the Bactrocera cucurbitae, is capable of amplifying a specific band of the length of 447bp, is incapable of amplifying the near-source species of the Bactrocera cucurbitae, and is high in amplification efficiency; the species specificity primer pair is simple to operate, fast and efficient; the species specificity primer pair is high in sensitivity, high in practical value, capable of fast, accurately and specifically detecting various insect states and even wrack of the Bactrocera cucurbitae, and large in detecting range.