Multiplex nucleic acid detecting method based on real-time fluorescence PCR (polymerase chain reaction) and probe method melting curve

Multiplex nucleic acid detecting method based on real-time fluorescence PCR (polymerase chain reaction) and probe method melting curve

The invention belongs to the field of biotechnology, and relates to a multiplex nucleic acid detecting probe and a detecting method, in particular to a multiplex nucleic acid detecting probe and a detecting method based on a real-time fluorescence PCR (polymerase chain reaction) technology and a pro...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YE XIANGZHONG, JI SHANGZHI, FU JUN, LI YIMIN
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention belongs to the field of biotechnology, and relates to a multiplex nucleic acid detecting probe and a detecting method, in particular to a multiplex nucleic acid detecting probe and a detecting method based on a real-time fluorescence PCR (polymerase chain reaction) technology and a probe method melting curve technology. The invention also relates to application of the probe or the method on multiplex nucleic acid detection. The probe which is designed according to a melting curve reaction program is added in a reaction system based on a real-time fluorescence PCR technology, the corresponding detecting method is provided, and the number of samples which can be detected in the same reaction system can be twice greater than the number of samples which can be detected on the basis of a real-time fluorescence PCR technology.