Functional Indel molecular marker primer A-2-1 of rice aroma gene Aro2 and application of molecular marker primer
The invention discloses a functional Indel molecular marker primer A-2-1 of a rice aroma gene Aro2. Nucleotide sequences of the molecular marker primer are shown as follows: forward primer: F: 5'-AAACAGGATAGAACTGGCTAC-3', and reverse primer R: 5'-CATTTACTGGGAGTTATGAAAC-3'; the pr...
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Zusammenfassung: | The invention discloses a functional Indel molecular marker primer A-2-1 of a rice aroma gene Aro2. Nucleotide sequences of the molecular marker primer are shown as follows: forward primer: F: 5'-AAACAGGATAGAACTGGCTAC-3', and reverse primer R: 5'-CATTTACTGGGAGTTATGAAAC-3'; the primers are subject to PCR amplification, and through polyacrylamide gel electrophoresis, a strip which is 211bp long is amplified from aromatic rice, a strip which is 219bp is amplified from non-aromatic rice, and strips which are 211bp and 219bp are simultaneously amplified from a heterozygous genotype rice material; the molecular marker primer A-2-1 is located within coding region of the Aro2 gene and is genetically isolated from aroma phenotype, and selection efficiency reaches 100%; and the molecular marker primer A-2-1 can be widely applied to the identification of aroma rice material and/or to aroma genotype selective breeding of later generations thereof. |
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