Method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil

The invention discloses a method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil. The method comprises the following steps: diluting waste undecanted wine, taking the diluted waste undecanted wine as the basic culture medium for oil-producing monoraphidium; adding inorganic salts into the basic culture medium, adjusting the pH value by NaOH to produce a culture medium; adding 250 mL of the obtained culture medium into a conical flask (500 mL), sterilizing, adding oil-producing monoraphidium, culturing monoraphidium; collecting the cells by a centrifugal separation method, extracting the grease by a chloroform/methanol extraction liquid, and finally adding 2 mL of sulfuric acid-methanol solution (3%) into the extract to carry out methyl esterification reactions so as to obtain the biodiesel oil. The provided method is simple and practical, is capable of largely reducing the culture time of oil-producing monoraphidium and production cost, and obviously increasing the grease yield, and thus the production efficiency of biodiesel oil is greatly improved.