Aeromonas sp. YQ and its application in enzymatic preparation of L-citrulline

The invention discloses aeromonas sp. YQ and its application in the enzymatic preparation of L-citrulline. The aeromonas sp. YQ is preserved in China Center for Type Culture Collection in Wuhan University in Wuhan, China under CCTCC No: M2015271 on 3th May, 2015, with postcode being 430072. The aero...

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Hauptverfasser:	DAI ANDI, ZHONG LI, ZHONG WEIHONG, QIU LEQUAN, WU SHIJIN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses aeromonas sp. YQ and its application in the enzymatic preparation of L-citrulline. The aeromonas sp. YQ is preserved in China Center for Type Culture Collection in Wuhan University in Wuhan, China under CCTCC No: M2015271 on 3th May, 2015, with postcode being 430072. The aeromonas sp. YQ obtained by screening is able to convert the substrate L-arginine to produce L-citrulline; whole-cells of the aeromonas sp. YQ are able to act for 3 days in pH 6 acetate buffer at 30 to 37 DEG C at a speed of 180r/min and able to convert 100g/L of the substrate L-arginine to produce 75g/L of L-citrulline, a product is about 47.8% in purity, the whole process has a total product yield of 40%, and basis is laid for industrialization of the enzymatic production of L-citrulline.