Bi-combined inactivate vaccine of aeromonas hydrophila and aeromonas veronii and preparation method thereof

The invention provides a bi-combined inactivate vaccine of aeromonas hydrophila and aeromonas veronii and a preparation method thereof and belongs to the field of bio-pharmacy. In the invention, with sodium alginate as a wall material and inactivated whole bacteria of the aeromonas hydrophila and the aeromonas veronii as a core material, the bi-combined oral slow-releasing microsphere vaccine of aeromonas hydrophila and aeromonas veronii is prepared through an emulsification method. By means of an aeromonas hydrophila and aeromonas veronii-calcium alginate micro-capsule oral vaccine for immunizing prussian carps, serum enzymatic activity and leukocyte phagocytic activity can be significantly improved. The vaccine is 46.7% in relative protection ratio on the prussian carps. The micro-capsule is stable in a simulated stomach-intestine environment and shows excellent properties of burst release and slow release, is excellent in maintenance of antigenicity of bacteria and is good in vaccine safety and storage stability.