Method for establishing mango stem aseptic strain system

Method for establishing mango stem aseptic strain system

The invention discloses a method for establishing a mango stem aseptic strain system, and relates to the field of plant tissue culture. The method comprises the following steps of: 1) scissoring mango twigs and stems, and pretreating the mango twigs and stems as explants; 2) sterilizing; 3) inoculat...

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Hauptverfasser: CEN XIANGTAO, NONG YANFENG, SU SHILIN
Format: Patent
Sprache:eng
Schlagworte:
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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creator CEN XIANGTAO
NONG YANFENG
SU SHILIN
description The invention discloses a method for establishing a mango stem aseptic strain system, and relates to the field of plant tissue culture. The method comprises the following steps of: 1) scissoring mango twigs and stems, and pretreating the mango twigs and stems as explants; 2) sterilizing; 3) inoculating; 4) carrying out incandescent light culture; 5) carrying out secondary sterilization; and 6) obtaining regenerated plants. According to the method, a conventional mode of obtaining mango regenerated plants by somatic embryogenesis is broken through; excellent characteristics of a mother plant can be inherited to obtain a great number of mango tissue culture seedlings; and germ-free shoots are induced by mango stem culture, so that intermediate propagation effect is achieved.
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subjects AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
title Method for establishing mango stem aseptic strain system
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