Method for amplifying Tianjin tip lamprotula F-type mitochondrial genome sequence

Method for amplifying Tianjin tip lamprotula F-type mitochondrial genome sequence

The invention relates to a method for amplifying the Tianjin tip lamprotula F-type mitochondrial genome sequence. The Tianjin tip lamprotula F-type mitochondrial genome sequence is obtained through total DNA extraction, common PCR amplification and sequencing, long-fragment primer designing, LA-PCR...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: AN CHANGTING, WU XIAOPING, ZHOU CHUNHUA, OUYANG SHAN, WU RUIWEN
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention relates to a method for amplifying the Tianjin tip lamprotula F-type mitochondrial genome sequence. The Tianjin tip lamprotula F-type mitochondrial genome sequence is obtained through total DNA extraction, common PCR amplification and sequencing, long-fragment primer designing, LA-PCR amplification and sequencing and sequence jointing; and the total length of the sequence is 15695bp, and the sequence comprises 13 protein genes, 22 tRNA genes, 2 rRNA genes and 1 FORF. The method for obtaining the Tianjin tip lamprotula F-type mitochondrial genome sequence is disclosed for the first time, and important base materials are provided for systemic embryology, comparison and evolutionary genomics, pedigree genomics, genetic resource conservation and sustainable and reasonable utilization of the unionidae family.