Method for obtaining bacterial strains of cheese starter
The invention discloses a method for bacterial strains of a cheese starter. The method comprises the following steps: inoculating Lactococcus lactis to a sodium chloride containing skim milk culture medium for culture, wherein the pH value of the skim milk culture medium is 4-5; separately inoculati...
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Zusammenfassung: | The invention discloses a method for bacterial strains of a cheese starter. The method comprises the following steps: inoculating Lactococcus lactis to a sodium chloride containing skim milk culture medium for culture, wherein the pH value of the skim milk culture medium is 4-5; separately inoculating the obtained bacterial strains to the skim milk culture medium for culture under different culture conditions, and selecting the bacterial strains which meet the certain requirement for the total number of living bacteria as Lactococcus lactis bacterial strains of type I; culturing the Lactococcus lactis bacterial strains of type I till curds are formed, and selecting Lactococcus lactis bacterial strains A of which the curd state is normal and the OD660 is larger than or equal to 0.7; inoculating Leuconostoc mesenteroides to the skim milk culture medium for culture, and selecting Leuconostoc mesenteroides bacterial strains B of which the curd state is normal and the aromatic flavor is strong; culturing the Lactococcus lactis bacterial strains A and the Leuconostoc mesenteroides bacterial strains B together. The method is high in repeatability and practical value, and very high in application value. |
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