Human cervical carcinoma metastasis relevant new long chain non-coding RNA sequence, separation method and uses thereof

The present invention relates to a human cervical carcinoma metastasis relevant new long chain non-coding RNA sequence, a target sequence for RNA interference, a short hairpin sequence for encoding interference target, a RNAi target sequence containing the long chain non-coding RNA sequence, a shRNA...

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Bibliographische Detailangaben
Hauptverfasser:	JIANG BINYUAN, QIN CHANGFEI, FANG SHUJUAN, PAN XI, LI YUEHUI, LI GUANCHENG, SUN RUILI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a human cervical carcinoma metastasis relevant new long chain non-coding RNA sequence, a target sequence for RNA interference, a short hairpin sequence for encoding interference target, a RNAi target sequence containing the long chain non-coding RNA sequence, a shRNA short hairpin nucleic acid sequence recombinant vector and a ecombinant. The present invention further relates to a separation method and uses of the new long chain non-coding RNA sequence. According to the present invention, the long chain non-coding RNA sequence is obtained from the cervical carcinoma related cDNA library through RACE amplification through a bioinformatics method, and the function and the uses of the RNA sequence are verified through sequencing, hybridization, cell proliferation, cell invasion and other tests; the sequence-specific shRNA for RNAi, the siRNA sequence and the recombinant vector are designed, and the functions of the siRNA and the shRNA are verified; and the long chain non-coding RNA sequence, the RNAi interference target and the shRNA short hairpin sequence provide important significance in early diagnosis and treatment of cervical carcinoma.