Method for pretreating lignocellulose raw material by combining ferric chloride and white rot fungi
The invention belongs to the technical field of lignocellulose raw material pretreatment and particularly discloses a method for pretreating a lignocelllose raw material by combining ferric chloride and white rot fungi. The method comprises the steps of (1) weighing a dry lignocellulose raw material...
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Format: | Patent |
Sprache: | eng |
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Zusammenfassung: | The invention belongs to the technical field of lignocellulose raw material pretreatment and particularly discloses a method for pretreating a lignocelllose raw material by combining ferric chloride and white rot fungi. The method comprises the steps of (1) weighing a dry lignocellulose raw material, adding a ferric chloride solution with the concentration of 0.1-0.5mol/L according to the solid-to-liquid ratio (g/ml) of 1/20 to 1/10, and treating at the temperature of 100-108 DEG C for 15-30 minutes after uniformly mixing; and (2) weighing the lignocellulose raw material treated by ferric chloride in the step (1) and washing to be neutral, adding distilled water according to the solid-to-liquid ratio (g/ml) of 1/6 to 1/4, sealing by using an aseptic sealing film, sterilizing at the temperature of 121+/-5 DEG C for 60+/-30 minutes, inoculating white rot fungi in a logarithmic phase, and culturing at the temperature of 28+/-3 for 6-30 days. According to the method, the lignocellulose raw material is sequentially treated by using ferric chloride and the white rot fungi, so that the content of revertose and the period are remarkably increased, and the hydrogen production capacity of the treated lignocellulose raw material is stronger. |
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