Multiplex DPO-PCR (dual-priming oligonucleotide-polymerase chain reaction) detection kit for sunflower white rust and black stem and application thereof

The invention discloses a multiplex DPO-PCR (dual-priming oligonucleotide-polymerase chain reaction) detection kit for sunflower white rust and black stem and application thereof. The multiplex DPO-PCR detection kit comprises a primer set for detecting or auxiliarily detecting whether the detected s...

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Hauptverfasser: SHANG SHUANG, WEI SHUANG, YIN JIEPING, LIANG QIAOLING, QIAN YIKE, YUAN JUNJIE, ZHANG NA, SAITIEERHAN, CHEN WEIMIN, LIU ZHONGYONG
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creator SHANG SHUANG
WEI SHUANG
YIN JIEPING
LIANG QIAOLING
QIAN YIKE
YUAN JUNJIE
ZHANG NA
SAITIEERHAN
CHEN WEIMIN
LIU ZHONGYONG
description The invention discloses a multiplex DPO-PCR (dual-priming oligonucleotide-polymerase chain reaction) detection kit for sunflower white rust and black stem and application thereof. The multiplex DPO-PCR detection kit comprises a primer set for detecting or auxiliarily detecting whether the detected strain is sunflower white rust or sunflower black stem, wherein the primer set is composed of a primer 1, a primer 2, a primer 3 and a primer 4. The test proves that the DPO primers and detection kit have the characteristics of favorable specificity, high accuracy and high sensitivity and can be used for detection, differentiation and identification of sunflower white rust and sunflower black stem.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Multiplex DPO-PCR (dual-priming oligonucleotide-polymerase chain reaction) detection kit for sunflower white rust and black stem and application thereof
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