Multiplex DPO-PCR (dual-priming oligonucleotide-polymerase chain reaction) detection kit for sunflower white rust and black stem and application thereof

The invention discloses a multiplex DPO-PCR (dual-priming oligonucleotide-polymerase chain reaction) detection kit for sunflower white rust and black stem and application thereof. The multiplex DPO-PCR detection kit comprises a primer set for detecting or auxiliarily detecting whether the detected s...

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Hauptverfasser:	SHANG SHUANG, WEI SHUANG, YIN JIEPING, LIANG QIAOLING, QIAN YIKE, YUAN JUNJIE, ZHANG NA, SAITIEERHAN, CHEN WEIMIN, LIU ZHONGYONG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a multiplex DPO-PCR (dual-priming oligonucleotide-polymerase chain reaction) detection kit for sunflower white rust and black stem and application thereof. The multiplex DPO-PCR detection kit comprises a primer set for detecting or auxiliarily detecting whether the detected strain is sunflower white rust or sunflower black stem, wherein the primer set is composed of a primer 1, a primer 2, a primer 3 and a primer 4. The test proves that the DPO primers and detection kit have the characteristics of favorable specificity, high accuracy and high sensitivity and can be used for detection, differentiation and identification of sunflower white rust and sunflower black stem.