Controllable porous vector-free immobilized lipase and preparation method thereof

The invention discloses a controllable porous vector-free immobilized lipase and a preparation method thereof. The preparation method of the controllable porous vector-free immobilized lipase comprises the following steps: coprecipitating lipase, calcium chloride, sodium carbonate and ammonium sulfate to prepare lipase/calcium carbonate microspheres, adding dithiothreitol for carrying out cross-linking and self-assembly of lipase, and then removing calcium carbonate by using ethylene diamine tetraacetic acid to prepare the controllable porous vector-free immobilized lipase. The preparation process of the controllable porous vector-free immobilized lipase is operated at normal temperature and pressure; the operation condition is mild; the prepared controllable porous vector-free immobilized lipase is stable in structure, controllable in shape and pore size, same in components of microparticles and high in enzyme activity, is capable of effectively reducing the mass transfer limitation of substrate molecules and improving the enzyme catalysis efficiency, and is suitable for popularization and application.