Preparation of impurity in cefetecol disodium and structure confirmation method

The invention discloses preparation of an impurity in cefetecol disodium and a structure confirmation method, and belongs to the pharmaceutical analysis technology field. Literature reports that a HPLC-MS method to carry out structure derivation of an impurity in a sample, but aimed to the impurity with a small content, satisfied results are difficult to obtain through the HPLC-MS method, and literature and patent reports related to the impurity are not discovered. Aimed to the above problem, separation is carried out by utilization of a preparation column, enrichment of the target impurity is carried out, further separation and purification are carried out, and finally the impurity monomer is obtained. Confirmation is carried out and the chemical structure of the impurity is obtained. The method is simple, the result is accurate, and the method can be used for impurity analysis control of cephalosporin samples.