In-vitro screening method for antineoplastic activity of artemisinin and derivatives of artemisinin

The invention discloses an in-vitro screening method for antineoplastic activity of artemisinin and derivatives of the artemisinin. The method comprises the following steps: analyzing the interaction between the artemisinin or the derivatives of the artemisinin and transferrin by adopting ultraviolet-visible absorption spectra or fluorescence spectra; calculating the combining factor Kb of the artemisinin or the derivatives of the artemisinin and the transferring; comparing the combining factor Kb of the artemisinin or the derivatives of the artemisinin and the transferring, or sequencing according to the combining factor Kb, and inferring the strength sequence of the antineoplastic activity of the artemisinin or the derivatives of the artemisinin; and verifying screening results by adopting antineoplastic activity tests. According to the method, a spectroscopic method is used for detecting the combining factor Kb of the artemisinin or the derivatives of the artemisinin and the transferring, and the in-vitro screening is carried out on the antineoplastic activity of the artemisinin and the derivatives of the artemisinin according to the sequence of the combining factor Kb. By using the method, the antineoplastic activity of the derivatives of the artemisinin can be fast and effectively judged, the method can be used for providing the base for designed synthesis of artemisinin-based drugs and clinical application of the artemisinin-based drugs, and promoting the application of the artemisinin-based drugs in the antineoplastic field.