RT-LAMP (reverse transcription loop-mediated isothermal amplification) reagent box detecting grapevine virus A and special primer thereof

The invention discloses an RT-LAMP (reverse transcription loop-mediated isothermal amplification) reagent box detecting grapevine virus A and a special primer thereof. The special primer of the RT-LAMP reagent box detecting grapevine virus A is composed of primer F3, primer B3, primer FIP, primer BI...

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Bibliographische Detailangaben
Hauptverfasser:	XIN YANYAN, LI GUIFEN, QIAN YIKE, ZHANG YONGJIANG
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an RT-LAMP (reverse transcription loop-mediated isothermal amplification) reagent box detecting grapevine virus A and a special primer thereof. The special primer of the RT-LAMP reagent box detecting grapevine virus A is composed of primer F3, primer B3, primer FIP, primer BIP, primer LF and primer LB. The nucleotide sequences include sequences 1 to 6 in a sequence table. Through experiment, the RT-LAMP reagent box and the special primer thereof can detect the grapevine virus A specially and have not cross reaction with other viruses, and the minimum detection limit of RNA of the grapevine virus A is 78.6 pg.