ISSR-SCAR (inter simple sequence repeat-sequence characterized amplified region) marker capable of identifying Wujiang brassica chinensis and identification method of marker

The invention discloses an ISSR-SCAR (inter simple sequence repeat-sequence characterized amplified region) marker capable of identifying Wujiang brassica chinensis and an identification method of the marker. The method includes: performing total DNA (deoxyribose nucleic acid) extraction, PCR (polym...

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Bibliographische Detailangaben
Hauptverfasser: LIN YIBO, CAO MINXU, ZHANG YANMEI, HANG YUEYU, SUN XIAOQIN, DAI HUAJUN, SHEN XUELIN
Format: Patent
Sprache:eng
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Zusammenfassung:The invention discloses an ISSR-SCAR (inter simple sequence repeat-sequence characterized amplified region) marker capable of identifying Wujiang brassica chinensis and an identification method of the marker. The method includes: performing total DNA (deoxyribose nucleic acid) extraction, PCR (polymerase chain reaction) amplification, primer selection and ISSR-SCAR detection on Wujiang brassica chinensis and other 10 brassica chinensis varieties, and finally determining an ISSR-SCAR molecular marker and a molecular identification method of the Wujiang brassica chinensis. A primer shown in the sequence such as SEQ ID NO:1 is used for amplification to obtain a specific fragment shown in the sequence such as SEQ ID NO:2. According to the sequence, a pair of primers in the sequences such as SEQ ID NO:3 and SEQ ID NO:4 is designed. The marker is simple to operate, stable and reliable, is the first molecular identification marker applied to the variety of Wujiang brassica chinensis, can be used for quickly identifying the variety and has significance of protecting germplasm resources of geographical indication products.