Optimized cell culture medium and cell culture method, and application of optimized cell culture medium and cell culture method to preparation of protein and antibody

The invention provides an optimized serum-free cell culture medium which is composed of a basal culture medium and additives, wherein the additives comprise glutamic acid with the final concentration of 2-5 mM and glutamine with the final concentration of 4 mM. The invention further provides the application of the cell culture medium to the culture of mammalian cells for production of therapeutic recombinant protein such as II type tumor necrosis factor receptor-antibody fusion protein (rhTNFRII-Fc)) and/or antibody such as CD20 monoclonal antibody drugs. By using the optimized cell culture medium for culturing mammalian cells, influences of metabolic by-products such as ammonium ions generated in the cell production culture process on the mammalian cell growing status and functions can be reduced and properties and yields of recombinant protein and the antibody are ensured.