Animal tissue genomic DNA rapid extraction kit, extraction method and application

The invention relates to an animal tissue genomic DNA rapid extraction kit, an extraction method and application. The rapid extraction kit includes a lysate, a wash solution 1, a wash solution 2, absolute ethyl alcohol and purified water. The extraction method includes the following steps: the solid...

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Hauptverfasser: YANG NING, ZHANG BIN, ZHANG LIXIAN, LI HAILI, YOU YI, XU ZHAOXUE, JIAO WENQIANG
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention relates to an animal tissue genomic DNA rapid extraction kit, an extraction method and application. The rapid extraction kit includes a lysate, a wash solution 1, a wash solution 2, absolute ethyl alcohol and purified water. The extraction method includes the following steps: the solid animal tissue is firstly grinded during extraction, and the liquid animal tissue is extracted directly; after the sample is split, the genomic DNA is adsorbed on a DNA adsorption column; then the purified water is used to wash to obtain the whole genome DNA. The kit can be used for simultaneous extraction and purification of genomic DNA of animal tissue; with the method, the whole extraction process only needs 5 minutes, the extraction efficiency is high, the detection sensitivity is high, and reliable experimental basis is provided for early diagnosis of various types of DNA bacteria or viral pathogens infection and early diagnosis; the animal tissue genomic DNA rapid extraction kit and the extraction method are applicable to all research institutions, clinical pathogen detection, molecular genetics research, clinical genetic diagnosis, and animal disease diagnosis and disease control centers, and have broad market prospects and greater economic benefits.