vip3A-1 gene of bacillus thuringiensis expression protein with high toxicity for asparagus caterpillar
The invention discloses a vip3A-1 gene of a bacillus thuringiensis expression protein with high toxicity for asparagus caterpillars, and belongs to the technical field of biochemistry. The expression protein is adopted for primary screening and secondary screening on bioactivity of asparagus caterpi...
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Zusammenfassung: | The invention discloses a vip3A-1 gene of a bacillus thuringiensis expression protein with high toxicity for asparagus caterpillars, and belongs to the technical field of biochemistry. The expression protein is adopted for primary screening and secondary screening on bioactivity of asparagus caterpillars, the number of dead and live asparagus caterpillars after 7 days, the death rate and LC50 are calculated and corrected, the result shows that the medial lethal concentration of the vip3A-1 gene of the bacillus thuringiensis expression protein to asparagus caterpillars is 7.755mu g/g (5.683-9.979mu g/g) in secondary screening, and when the concentration of the expression protein is up to 81mu g/g, all asparagus caterpillars can be dead, so that the vip3A-1 gene of the bacillus thuringiensis expression protein has high toxicity for asparagus caterpillars, and is characterized in that the nucleotide sequence of the vip3A-1 gene of the bacillus thuringiensis expression protein with high toxicity for asparagus caterpillars is as shown in SEQ ID NO:1. |
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