Method for separating and determining chromium elements with different valences in tobacco and tobacco products

The invention discloses a method for separating and determining chromium elements with different valences in tobacco and tobacco products. The method comprises the following steps of 1, selecting apparatuses and equipment, wherein the separation and determination device is a high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) combined device, 2, preparing a reagent and a standard solution, 3, pretreating a sample, 4, determining the sample and calculating and expressing a calculation result. The method utilizes the HPLC-ICP-MS combined technology to realize separation and determination of chromium elements with different valences in tobacco and tobacco products, has good effects of analysis, determination and separation of chromium elements with different valences, a low detecting limit, high sensitivity and good repeatability, can detect chromium elements with different valences in a sub-concentration (microgram/liter) level, and in other word, can detect content of Cr(III) and Cr(VI) in tobacco and tobacco products. The method for separation and determination optimizes a sample pre-treatment process and simplifies separation and determination processes.