Cotton GhEDR2 gene as well as encoding protein and application of cotton GhEDR2 gene

The invention relates to plant gene cloning and functional analysis belonging to the field of plant genetic engineering and particularly relates to a cotton GhEDR2 gene as well as an encoding protein and application of the cotton GhEDR2 gene. A cotton EST fragment which is homologous with the cDNA o...

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Bibliographische Detailangaben
Hauptverfasser:	LUO XIAOLI, WANG ZHIAN, XIAO JUANLI, ZHANG ANHONG
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to plant gene cloning and functional analysis belonging to the field of plant genetic engineering and particularly relates to a cotton GhEDR2 gene as well as an encoding protein and application of the cotton GhEDR2 gene. A cotton EST fragment which is homologous with the cDNA of a disease-resistant gene of Arabidopsis thaliana is found according to the relative conservative property of homologous gene sequences, partially-overlapped cotton EST with high homology is subjected to sequence splitting to and combined to form cDNA sequences of complete ORF, a specific PCR primer is designed according to the sequences, and then, the GhEDR2 gene is obtained by using an RT-PCR method. Found through qPCR, the expression of the gene in a cotton plant has the characteristic of tissue specificity. In addition, the morbidity of verticillium wilt of transgenic plants is obviously lower than that of non-transgenic plants. Therefore, the GhEDR2 gene disclosed by the invention has a wide prospect in plant breeding for disease resistance when being used for culturing plants resistant to verticillium wilt.