Phellinus linteus polysaccharide separation and purification method

The invention discloses a Phellinus linteus polysaccharide separation and purification method which is characterized by mainly comprising the following steps: preparing a Phellinus linteus polysaccharide component by an ammonium oxalate extraction-ethanol precipitation process, carrying out anion exchange chromatography and gel filtration chromatography to separate and purify the polysaccharide, and carrying out concentration, dialysis, vacuum freeze drying and the like on the product to obtain the Phellinus linteus polysaccharide pure product. The method does not influence the chemical structure of the natural polysaccharide, and can keep the original bioactivity of the natural polysaccharide. The method has the advantages of low requirements for equipment and environment, simple process and low cost, is flexible and practical, and is beneficial to popularization, development and application.