Preparation method of acellular bionet and acellular bionet prepared by preparation method

The invention discloses a preparation method of a acellular bionet and the acellular bionet prepared by the preparation method. The preparation method of the acellular bionet comprises the step of acellular disposal by immersing omentum majus in a buffer solution containing lauryl sodium sulfate and DNA enzyme. The invention also discloses the acellular bionet prepared by the above method. By combining gradient elution and physical oscillation, the method has advantages as follows: conditions are mild; acellular efficiency of omentum majus is enhanced; and composition of omentum majus acellular matrix is retained to the maximum. The method also has advantages of simple operation and strong enforceability.