Preparation method of foot-and-mouth disease vaccines

Preparation method of foot-and-mouth disease vaccines

The invention provides a preparation method of foot-and-mouth disease vaccines. The method comprises the following steps: (1) filtering a cell culture fluid of viruses by using a deep filter device at a flow velocity of 300-500 L/m /hr; (2) carrying out ultrafiltration and concentration on antigen l...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: ZHANG TAO, HE ZHAOQING, LIU YANTAO, WANG YONGWEI, ZHANG SHU, ZHAO YINGHUI, LI RUHENG, KANG BIN, ZHAO BINGWU, TIAN PUHOU
Format: Patent
Sprache:eng
Schlagworte:
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
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Beschreibung
Zusammenfassung:The invention provides a preparation method of foot-and-mouth disease vaccines. The method comprises the following steps: (1) filtering a cell culture fluid of viruses by using a deep filter device at a flow velocity of 300-500 L/m /hr; (2) carrying out ultrafiltration and concentration on antigen liquid by using a hollow fiber column or membrane envelope; (3) enabling a virus antigen obtained by using the purification and concentration method to pass through DEAE-Sepharose FF and Sephawse6FF two-step chromatographic columns; (4) carrying out inactivation by using BEI, and blocking by using sodium thiosulfate; and (5) carrying out emulsification by using a 206 adjuvant. Due to vaccines prepared by using the method, immune animals can achieve a good immune effect, less adverse reaction and high safety.