Method for preparing folic acid functional nanofibers for target capture of cancer cells

The invention relates to a method for preparing folic acid functional nanofibers for target capture of cancer cells. The method comprises the following steps: (1) preparing a PVA/PEI nanofiber membrane by electrostatic spinning, and carrying out vacuum drying; (2) after drying, using glutaraldehyde...

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Bibliographische Detailangaben
Hauptverfasser:	SHI XIANGYANG, FAN ZHANGYU, ZHAO YILI
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBONFILAMENTS BRAIDING CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS,THREADS, FIBRES, BRISTLES OR RIBBONS COTTON-WOOL FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS,NON-WOVEN FABRICS FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR KNITTING LACE-MAKING LAUNDERING MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARYMATERIAL MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OFARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES ORRIBBONS NATURAL OR ARTIFICIAL THREADS OR FIBRES NON-WOVEN FABRICS SPINNING TEXTILES TREATMENT OF TEXTILES OR THE LIKE TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS,FABRICS, FEATHERS, OR FIBROUS GOODS MADE FROM SUCH MATERIALS TRIMMINGS WADDING
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Beschreibung
Zusammenfassung:	The invention relates to a method for preparing folic acid functional nanofibers for target capture of cancer cells. The method comprises the following steps: (1) preparing a PVA/PEI nanofiber membrane by electrostatic spinning, and carrying out vacuum drying; (2) after drying, using glutaraldehyde for crosslinking; (3) activating carboxyl of folic acid FA through EDC.HCl and NHS, and reacting the carboxyl-activated FA with polyethylene glycol PEG with carboxyl at one end and amidogen at the other end to obtain FA-PEG-COOH; (4) activating carboxyl of FA-PEG-COOH, adding FA-PEG-COOH into the PVA/PEI nanofiber membrane, and carrying out shaking reaction to obtain the folic acid functional nanofibers; adding triethylamine and acetic anhydride for acetylation treatment, washing and drying. The prepared nanofibers have good biocompatibility and structural stability, is short in time to capture the cancer cells and strong in specificity, and has a wide application prospect.