Primers for detecting anti-sulfonylurea herbicide gene BnALS3R of cabbage type rape and application of primer

The invention discloses primers for detecting an anti-sulfonylurea herbicide gene BnALS3R of cabbage type rape and the application of the primers, and belongs to the field of molecular breeding. A method for detecting the anti-sulfonylurea herbicide gene BnALS3R of the cabbage type rape by use of th...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG JIEFU, ZHOU XIAOYING, PU HUIMING, CHEN SONG, CHEN FENG, PENG QI, WANG XIAODONG, QI CUNKOU, HU MAOLONG, FU SANXIONG, LONG WEIHUA, ZHANG WEI, GAO JIANQIN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses primers for detecting an anti-sulfonylurea herbicide gene BnALS3R of cabbage type rape and the application of the primers, and belongs to the field of molecular breeding. A method for detecting the anti-sulfonylurea herbicide gene BnALS3R of the cabbage type rape by use of the primers SEQ ID NO.1 and SEQ ID NO.2 comprises the following steps: taking the total DNA of the cabbage type rape as a template and adding a primer for PCR amplification; performing restriction enzyme BsrDI digestion on the PCR product; differentiating homozygous rape containing the resistance gene BnALS3R, hybrid rape containing the resistance gene BnALS3R, and homozygous rape not containing the resistance gene BnALS3R by use of the enzyme digestion product. According to the primers, whether rape planets contain the resistance gene BnALS3R can be detected quickly and accurately, and whether the contained anti-herbicide gene is a homozygous type or a hybrid type also can be detected accurately, and therefore, the breeding objective of breeding a new anti-herbicide rape variety can be achieved quickly and effectively.