Swine enterotoxigenic Excherichia coli flagellin 3FliCon fusion protein and application thereof

The invention discloses a swine enterotoxigenic Excherichia coli flagellin 3FliCon fusion protein and an application thereof, and provides a recombinant plasmid pRSET-3FliCon containing 3 copy genes fliC of swine ETC. The recombinant plasmid is converted to Escherichia coli BL21(DE3) to induce a rec...

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Bibliographische Detailangaben
Hauptverfasser:	PENG JIAN, WANG RONG, JIANG SIWEN, PAN ZHONGMIAN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a swine enterotoxigenic Excherichia coli flagellin 3FliCon fusion protein and an application thereof, and provides a recombinant plasmid pRSET-3FliCon containing 3 copy genes fliC of swine ETC. The recombinant plasmid is converted to Escherichia coli BL21(DE3) to induce a recombinant expression bacterium BL21(DE3)/pREST-3FliCon to express the fusion protein 3FliCon fusion. The above expressed recombinant protein is used to actively immunize New Zealand male rabbits, a serum antibody aiming at the protein is prepared, the recombinant protein has good antigenicity is verified, an intestinal epithelial cell line IPEC-J2 is cultured, and an adherence inhibition test is used to prove that swine ETEC flagellum plays a great role in the adherence process of small intestinal epithelial cells. A chicken yolk antibody prepared by using the fusion protein can inhibit the adherence of epithelial cells to the ETEC in vitro, and can inhibit ETEC strains of different serum types.