Method for obtaining tissue culture plantlets fast by utilizing homogenate of stems and leaves of hydrilla verticillata

The invention relates to a method for obtaining tissue culture plantlets fast by utilizing the homogenate of stems and leaves of hydrilla verticillata, and belongs to the technical field of plant tissue culture. The method comprises the following steps: carrying out asepsis on different explants of hydrilla verticillata; after pre-culture, conducting induction and culture on calluses of the precultured explants; transferring the callus into a propagation medium, so as to form a green globular body (GGB); conducting twice differentiation culture on the GGB, so as to obtain plantlets and new calluses; conducting rooting culture on the plantlets; conducting propagation culture on the new calluses to form GGB; in cycles, obtaining a large quantity of tissue culture plantlets. The method has the benefits that the pollution rate of the explants is low; browning of the explants is avoided, so that fast propagation effect of hydrilla verticillata is realized; new tissue culture plantlets can be obtained through direct differentiation, so that the propagation cycle of hydrilla verticillata is greatly shortened, convenience is brought for mass production in a plant, and a technical effect with a high propagation coefficient is formed; different illumination intensities are adopted to avoid vitrification of the calluses and differentiated seedlings, so that the culture effect is excellent.