Construction method of fingerprint of Ilex rotunda thunb medicinal material and detection method of Ilex rotunda thunb medicinal material

The invention discloses a construction method of a fingerprint of Ilex rotunda thunb medicinal material and a detection method of the Ilex rotunda thunb medicinal material. The construction method comprises the following steps: (1) preparation of a reference solution; (2) preparation of a test solution; and (3) determination, namely respectively precisely sucking up the test solution and the reference solution, and injecting the sucked solutions into a high performance liquid chromatograph for determination, thereby obtaining the fingerprint of Ilex rotunda thunb medicinal material composed of 13 common characteristic peaks. For the construction method and the detection method, through a lot of experiments and research by the inventor, the method for preparing the test solution and the reference solution is determined, optimal parameters of chromatographic conditions of the high performance liquid chromatograph are also determined, and therefore, the fingerprint of the Ilex rotunda thunb medicinal material is constructed. With the fingerprint constructed by the method disclosed by the invention and the detection method, the Ilex rotunda thunb medicinal material can be identified accurately, precision is high, and a reagent is saved.