Method for establishing lipopolysaccharide (LPS)-induced milk cow mammary gland epithelial cell apoptosis model

Method for establishing lipopolysaccharide (LPS)-induced milk cow mammary gland epithelial cell apoptosis model

The invention discloses a method for establishing a lipopolysaccharide (LPS)-induced milk cow mammary gland epithelial cell apoptosis model, which comprises the following steps: (1) acquiring 2nd-3rd-generation milk cow mammary gland epithelial cells; (2) detecting inhibiting action of LPS on milk c...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: FU KUN, HUANG LONG, WANG YUANLANG, ZHU SHUJIAO, ZHANG XIAODONG, ZHANG MENGQI, YIN ZONGJUN, MENG YUN, DING YUEYUN, ZHANG WEI
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention discloses a method for establishing a lipopolysaccharide (LPS)-induced milk cow mammary gland epithelial cell apoptosis model, which comprises the following steps: (1) acquiring 2nd-3rd-generation milk cow mammary gland epithelial cells; (2) detecting inhibiting action of LPS on milk cow mammary gland epithelial cell proliferation by a CCK-8 process, and primarily detecting the LPS action concentration and action time which conform to the requirement that the apoptosis rate of the established in-vitro cell trauma model is 40-50%; (3) detecting the mammary gland epithelial cell apoptosis rate by flow cytometry; (4) detecting variation conditions of apoptosis cell nucleic acids by DNA ladder; and (5) observing the interior morphological structure variation of the mammary gland epithelial cells by a transmission electron microscope. The determination method of the LPS-induced milk cow mammary gland epithelial cell apoptosis model induction conditions has the advantages of ideal effect and low cost, and is convenient to operate.