Tissue culture method of Koelreuteria bipinnata var. Integrifoliola ''Jinyan'

The invention relates to the technical field of plant tissue culture and discloses a tissue culture method of Koelreuteria bipinnata var. integrifoliola ''Jinyan''. The method includes the steps of pretreatment, primary culture, subculture and transplanting. Each growth stage uses a corresponding medium mainly comprising major elements, trace elements, ferric salt, organic additives and a plant growth regulator. The the external conditions are controlled at pH value of 5.8-6.0, culture temperature of 23-25 DEG C, illumination period of 8-10 h/d and illumination intensity of1800-2000 lux. The invention has the beneficial effects that the tissue culture for asexual reproduction of Koelreuteria bipinnata var. integrifoliola ''Jinyan'' not only has high breeding rate and high survival rate but also ensures that the culture plant does not show segregation of character to maintain the excellent characteristics of colorful leaves.