Primers and method for specific loop-mediated isothermal amplification (LAMP) detection of genetically modified alfalfa strain J163 and application of method

The invention discloses primers and method for specific loop-mediated isothermal amplification (LAMP) detection of a genetically modified alfalfa strain J163 and an application of the method. The sequences of the primers are respectively shown in SEQ ID NO.1- SEQ ID NO.6. Aiming at the genetically m...

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Hauptverfasser:	SU CAIZHU, ZHENG GAOBIN, QIN ZHUOMIN, FAN WUJIANG, FU QIJIAO, LYU YINGZI, WANG DINGGUO, YAO BAIHUI, JIANG XIANG, LIN HUIJIAO, LIU ERLONG, TANG JIE, LU LI, LIN XUEQIN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses primers and method for specific loop-mediated isothermal amplification (LAMP) detection of a genetically modified alfalfa strain J163 and an application of the method. The sequences of the primers are respectively shown in SEQ ID NO.1- SEQ ID NO.6. Aiming at the genetically modified alfalfa strain J163 from the Monsanto company to which the ministry of agriculture of China has not issued a security certificate of genetically modified organisms in agriculture, the specific LAMP rapid detection method of the alfalfa strain J163 is successfully established by designing a group of specific primers in an adjacent zone between a 5' terminal exogenous insert fragment P-eFMV (promoter) of the genetically modified alfalfa strain J163 and alfalfa genome DNA and promoting LAMP with BstDNA polymerase. The detection method is simple to operate, has strong specificity and high sensitivity, has a minimum detection copy number of 10 and can be used for carrying out detection analysis and identification on the components of genetically modified alfalfa J163 rapidly and accurately.