Method for separating and extracting hyaluronic acid (HA) from rana chensinensis skins by using membrane filtration process

The invention discloses a method for separating and extracting hyaluronic acid (HA) from rana chensinensis skins by using a membrane filtration process, and belongs to the field of methods for separating and extracting HA. According to the method disclosed by the invention, modern biological separation measures and methods such as membrane filtration are adopted, a ceramic membrane of which the membrane pore is 500nm is selected, the molecular weight cut off of the ceramic membrane is 1,000,000 Daltons, and thus protein can be effectively removed and HA can be intercepted. According to the method for extracting HA from the rana chensinensis skins, disclosed by the invention, 2.8g of high-quality HA can be extracted from per kilogram of the rana chensinensis skins, and by virtue of inspection, the glucuronic acid content of a product is 36%, and the protein content is 0.58%, so that the product can reach a cosmetic grade. According to the method, protein is removed by using the membrane filtration process, HA is desalted, graded and concentrated, high yield can be achieved, very little damage to the biological activity of polysaccharides can be caused, and the problem of reagent residues existing in a conventional organic solvent method also cannot be caused, so that the method is an economic, simple, convenient and effective extraction process and is suitable for industrial production of HA.