Method for separating polyphenol monomer from canavalia maritime by adopting high-speed countercurrent chromatography

The invention discloses a method for separating a polyphenol monomer from canavalia maritime by adopting high-speed countercurrent chromatography. The method comprises the following steps; (1) extracting canavalia maritime by using an accelerated solvent extraction method to obtain a coarse extract; (2) selecting a solvent system of the high-speed countercurrent chromatography; (3) concentrating the coarse extract to a paste, dissolving by using a bottom phase of the solvent system of the high-speed countercurrent chromatography to obtain a high-speed countercurrent chromatographic separation sample liquid; (4) separating sample liquid by the high-speed countercurrent chromatography and collecting fractions; and (5) analyzing the fractions by using high performance liquid chromatography, qualitatively determining the fractions according to retention time during the high performance liquid chromatography, and collecting the fraction which is consistent in retention time with main components of the coarse extract to obtain the polyphenol monomer. The method combines accelerated solvent extraction ASE and high speed countercurrent chromatography HSCCC to separate and purify the polyphenol monomer from canavalia maritime. Compared with the conventional separation and purification methods, the method has the advantage that the precision and reproducibility are improved. The method is simple to operate, small in time consumption, wide in application range, small in environmental pollution and easy to industrially promote.