Method for fast detecting salmonella in fresh milk

The invention relates to a method for fast detecting salmonella in fresh milk and belongs to the technical field of microbiological detection. The method includes: adding a fresh milk sample into buffer solution to obtain fresh milk fixture, wherein the buffer solution contains reagent for removing protein and fat; filtering the fresh milk mixture with filter paper, and using a micro-pore filter membrane to perform extraction filtration on filtrate; cutting the micro-pore filter membrane into pieces after filtration, and adding tetrathionate broth and phosphate buffer solution for washing and soaking to obtain bacterium suspension; adding salmonella immunomagnetic beads into the bacterium suspension, mixing, oscillating, standing on a magnetic rack for layering, and abandoning supernatant; taking lower-layer solution, adding phosphate buffer solution for washing, standing on the magnetic rack for layering, and abandoning the supernatant to obtain concentrated to-be-detected solution; using an Elisa detecting method to detect the to-be-detected solution. By the method, the total salmonella detecting time is 50 minutes, and detecting time is shortened greatly as compared with a national standard method.