L-glutamate oxidase gene from streptomyces diastatochromogenes as well as preparation method and application thereof

The invention relates to an L-glutamate oxidase gene from streptomyces diastatochromogenes as well as a preparation method and application thereof and in particular relates to a recombinant bacterium which is obtained by cloning and coding the L-glutamate oxidase gene from streptomyces diastatochrom...

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Bibliographische Detailangaben
Hauptverfasser:	GAO JIANJIE, DING WEIXING, YAO QUANHONG, YUAN ZHONGYI, PENG RIHE, YAN PEILAN, WANG LIJUAN, WANG RONGTAN, ZHU BO, WANG BO, LI DAWEI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to an L-glutamate oxidase gene from streptomyces diastatochromogenes as well as a preparation method and application thereof and in particular relates to a recombinant bacterium which is obtained by cloning and coding the L-glutamate oxidase gene from streptomyces diastatochromogenes screened from the soil and artificially synthesizing the gene and recombining the gene in an escherichia coli and has the activity of L-glutamate oxidase. The nucleotide sequence of the L-glutamate oxidase gene is SEQ ID No.1, the full length of the L-glutamate oxidase gene is 1974bp, and a coding protein sequence of the L-glutamate oxidase gene is SEQ ID No.2. L-glutamate oxidase coded by the gene can be used for specifically catalyzing L-glutamic acid, can serve as an enzyme for detecting the content of L-glutamic acid and is applied to the fields of the food industry and clinical biochemical detection.