SS-PCR (sequence specific primers-polymerase chain reaction) specific primers and kit for quickly identifying pumpkin fruit-fly

The invention relates to the field of quarantine pest detection, particularly an SS-PCR (sequence specific primers-polymerase chain reaction) specific primer pair and kit for quickly identifying pumpkin fruit-fly. The primer pair comprises a primer NF404: TGCCTTAGCGATATTCTGGCT and a primer NR610:AAC...

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Hauptverfasser: LIN YANGWU, CHEN SHAOPING, HUANG ZHEN, SHEN JIANGUO, LOU DINGFENG, GUO QIONGXIA
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creator LIN YANGWU
CHEN SHAOPING
HUANG ZHEN
SHEN JIANGUO
LOU DINGFENG
GUO QIONGXIA
description The invention relates to the field of quarantine pest detection, particularly an SS-PCR (sequence specific primers-polymerase chain reaction) specific primer pair and kit for quickly identifying pumpkin fruit-fly. The primer pair comprises a primer NF404: TGCCTTAGCGATATTCTGGCT and a primer NR610:AACTGTGTTCAGCAGGTGGT. The SS-PCR method for quickly identifying pumpkin fruit-fly is quick and simple, has the advantages of high specificity, high sensitivity and the like, can complete pumpkin fruit-fly identification within 8 hours, and can satisfy the requirements for seaport inspection/quarantine quick clearance.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title SS-PCR (sequence specific primers-polymerase chain reaction) specific primers and kit for quickly identifying pumpkin fruit-fly
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